In the meselson-stahl experiment, which mode of replication was eliminated based on data derived after one generation of replication?

a. conservative
b. semiconservative
c. dispersive
d. none of the modes

Answers

Conservative mode of replication

Explanation:

Meselson and Stahl performed the experiment to test how the DNA molecule replicates in the organisms.

He performed the experiment on E. coli by culturing them in N¹⁵ isotope and then transferring them to N¹⁴ isotope.

When he analysed the DNA sample through density centrifugation he found that in Generation One or one round of replication the intermediate band between the N¹⁴ isotope (lighter) and N¹⁵ isotope (heavier) isotopes.

This result showed that now the DNA contains both forms of isotope that is it is in favour of the semi-conservative and dispersive mode of replication but not conservative mode pf replication.

Thus, Conservative mode of replication is the correct answer.

CONSERVATIVE

Explanation:MESELSON-STAHL EXPERIMENT is an experiment carried out by Matthew Meselson and Franklin Stahl in the year 1958 which supported Watson and Crick's hypothesis that DNA replication was semiconservative.

After the completion of the first replication cycle,it was discovered that the replication is not a complete conservative process but a semi-conservative in nature. It shows that when DNA replicates one of the Helical strand is from the

one strand from the original or old helix and one newly synthesized helix.

CONSERVATIVE

Explanation:MESELSON-STAHL EXPERIMENT is an experiment carried out by Matthew Meselson and Franklin Stahl in the year 1958 which supported Watson and Crick's hypothesis that DNA replication was semiconservative.

After the completion of the first replication cycle,it was discovered that the replication is not a complete conservative process but a semi-conservative in nature. It shows that when DNA replicates one of the Helical strand is from the

one strand from the original or old helix and one newly synthesized helix.

conservative model

Explanation: DNA isolated after one generation produced a single band which was a higher and intermediate in density between the heavy and light Nitrogen isotopic DNA used, showing that the first generation was a hybrid DNA. This observation fit with the dispersive and semi-conservative models only. And so at this generation, the conservative model was discarded.

conservative model

Explanation: DNA isolated after one generation produced a single band which was a higher and intermediate in density between the heavy and light Nitrogen isotopic DNA used, showing that the first generation was a hybrid DNA. This observation fit with the dispersive and semi-conservative models only. And so at this generation, the conservative model was discarded.



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